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Bradyzoite Stages of Toxoplasma gondii in mouse brain stained with Giemsa in different treatment groups (n = 6 samples/group); scale bar = 20μm. ( a ) Bradyzoite in the infected-untreated group. ( b ) Bradyzoite in the <t>Spiramycin</t> treated group. ( c ) Bradyzoite in the CSNPs treated group. ( d ) Bradyzoites in the Cur-CSNPs treated group exhibited a significant reduction in cyst size ( P = 0.001) compared to other treated groups, whereas no statistically significant difference was observed between other groups ( P > 0.05).
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Bradyzoite Stages of Toxoplasma gondii in mouse brain stained with Giemsa in different treatment groups (n = 6 samples/group); scale bar = 20μm. ( a ) Bradyzoite in the infected-untreated group. ( b ) Bradyzoite in the <t>Spiramycin</t> treated group. ( c ) Bradyzoite in the CSNPs treated group. ( d ) Bradyzoites in the Cur-CSNPs treated group exhibited a significant reduction in cyst size ( P = 0.001) compared to other treated groups, whereas no statistically significant difference was observed between other groups ( P > 0.05).
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Bradyzoite Stages of Toxoplasma gondii in mouse brain stained with Giemsa in different treatment groups (n = 6 samples/group); scale bar = 20μm. ( a ) Bradyzoite in the infected-untreated group. ( b ) Bradyzoite in the <t>Spiramycin</t> treated group. ( c ) Bradyzoite in the CSNPs treated group. ( d ) Bradyzoites in the Cur-CSNPs treated group exhibited a significant reduction in cyst size ( P = 0.001) compared to other treated groups, whereas no statistically significant difference was observed between other groups ( P > 0.05).
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Bradyzoite Stages of Toxoplasma gondii in mouse brain stained with Giemsa in different treatment groups (n = 6 samples/group); scale bar = 20μm. ( a ) Bradyzoite in the infected-untreated group. ( b ) Bradyzoite in the <t>Spiramycin</t> treated group. ( c ) Bradyzoite in the CSNPs treated group. ( d ) Bradyzoites in the Cur-CSNPs treated group exhibited a significant reduction in cyst size ( P = 0.001) compared to other treated groups, whereas no statistically significant difference was observed between other groups ( P > 0.05).
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Bradyzoite Stages of Toxoplasma gondii in mouse brain stained with Giemsa in different treatment groups (n = 6 samples/group); scale bar = 20μm. ( a ) Bradyzoite in the infected-untreated group. ( b ) Bradyzoite in the Spiramycin treated group. ( c ) Bradyzoite in the CSNPs treated group. ( d ) Bradyzoites in the Cur-CSNPs treated group exhibited a significant reduction in cyst size ( P = 0.001) compared to other treated groups, whereas no statistically significant difference was observed between other groups ( P > 0.05).

Journal: Scientific Reports

Article Title: Exploring the role of chitosan and curcumin-loaded chitosan nanoparticles against chronic toxoplasma infection in experimental mice

doi: 10.1038/s41598-025-25252-5

Figure Lengend Snippet: Bradyzoite Stages of Toxoplasma gondii in mouse brain stained with Giemsa in different treatment groups (n = 6 samples/group); scale bar = 20μm. ( a ) Bradyzoite in the infected-untreated group. ( b ) Bradyzoite in the Spiramycin treated group. ( c ) Bradyzoite in the CSNPs treated group. ( d ) Bradyzoites in the Cur-CSNPs treated group exhibited a significant reduction in cyst size ( P = 0.001) compared to other treated groups, whereas no statistically significant difference was observed between other groups ( P > 0.05).

Article Snippet: Spiramycin® tablets (Rovamycin® -Sanofi Aventis Pharmaceutical Company, France) were weighed and crushed into powder.

Techniques: Staining, Infection

Photomicrograph of brain tissue sections (n = 6 samples/group) stained with hematoxylin and eosin from Spiramycin-treated group ( A , B ) demonstrating congested blood vessel (curved arrow), and neuronal degeneration (arrow), CSNPs-treated group ( C , D ) demonstrating tissue cyst (square), congested blood vessel (asterisk), and neuronal degeneration (arrow), inset is high magnification, and Cu-CSNPs-treated group ( E , F ) demonstrating apparently normal appearance of neurons (arrow) and glia cells (curved arrow). CSNPs: Chitosan nanoparticles-treated group; Cur-CSNPs: curcumin-loaded Chitosan nanoparticles-treated group.

Journal: Scientific Reports

Article Title: Exploring the role of chitosan and curcumin-loaded chitosan nanoparticles against chronic toxoplasma infection in experimental mice

doi: 10.1038/s41598-025-25252-5

Figure Lengend Snippet: Photomicrograph of brain tissue sections (n = 6 samples/group) stained with hematoxylin and eosin from Spiramycin-treated group ( A , B ) demonstrating congested blood vessel (curved arrow), and neuronal degeneration (arrow), CSNPs-treated group ( C , D ) demonstrating tissue cyst (square), congested blood vessel (asterisk), and neuronal degeneration (arrow), inset is high magnification, and Cu-CSNPs-treated group ( E , F ) demonstrating apparently normal appearance of neurons (arrow) and glia cells (curved arrow). CSNPs: Chitosan nanoparticles-treated group; Cur-CSNPs: curcumin-loaded Chitosan nanoparticles-treated group.

Article Snippet: Spiramycin® tablets (Rovamycin® -Sanofi Aventis Pharmaceutical Company, France) were weighed and crushed into powder.

Techniques: Staining

Photomicrograph of liver tissue sections (n = 6 samples/group) stained with hematoxylin and eosin from Spiramycin-treated group ( A , B ) demonstrating slight infiltration of mononuclear cells in portal areas (arrow), CSNPs-treated group ( C , D ) demonstrating tissue cysts (wavy arrow) and vascular congestion (asterisk) with perivascular inflammatory cells infiltration (arrow), and Cu-CSNPs-treated group ( E , F ) demonstrating normal structure of the hepatic parenchyma with mild vascular congestion (asterisk) in some examined lobules. CSNPs: Chitosan nanoparticles-treated group; Cur-CSNPs: curcumin-loaded Chitosan nanoparticles-treated group.

Journal: Scientific Reports

Article Title: Exploring the role of chitosan and curcumin-loaded chitosan nanoparticles against chronic toxoplasma infection in experimental mice

doi: 10.1038/s41598-025-25252-5

Figure Lengend Snippet: Photomicrograph of liver tissue sections (n = 6 samples/group) stained with hematoxylin and eosin from Spiramycin-treated group ( A , B ) demonstrating slight infiltration of mononuclear cells in portal areas (arrow), CSNPs-treated group ( C , D ) demonstrating tissue cysts (wavy arrow) and vascular congestion (asterisk) with perivascular inflammatory cells infiltration (arrow), and Cu-CSNPs-treated group ( E , F ) demonstrating normal structure of the hepatic parenchyma with mild vascular congestion (asterisk) in some examined lobules. CSNPs: Chitosan nanoparticles-treated group; Cur-CSNPs: curcumin-loaded Chitosan nanoparticles-treated group.

Article Snippet: Spiramycin® tablets (Rovamycin® -Sanofi Aventis Pharmaceutical Company, France) were weighed and crushed into powder.

Techniques: Staining

Photomicrograph of spleen tissue sections (n = 6 samples/group) stained with hematoxylin and eosin from negative control ( A – C ), infected-untreated ( D – F ), Spiramycin-treated ( G – I ), CSNPs-treated ( J – L ), and Cur-CSNPs-treated groups ( M – O ). (RP) red pulp, (WP) white pulp, (M) megakaryocytes, (arrow) lymphoid necrosis, (curved arrow) lymphoid depletion, (arrowhead) congestion, (wavy arrow) perifollicular hemorrhage, and (asterisk) hemorrhage in red pulp. CSNPs: Chitosan nanoparticles-treated group; Cur-CSNPs: curcumin-loaded Chitosan nanoparticles-treated group.

Journal: Scientific Reports

Article Title: Exploring the role of chitosan and curcumin-loaded chitosan nanoparticles against chronic toxoplasma infection in experimental mice

doi: 10.1038/s41598-025-25252-5

Figure Lengend Snippet: Photomicrograph of spleen tissue sections (n = 6 samples/group) stained with hematoxylin and eosin from negative control ( A – C ), infected-untreated ( D – F ), Spiramycin-treated ( G – I ), CSNPs-treated ( J – L ), and Cur-CSNPs-treated groups ( M – O ). (RP) red pulp, (WP) white pulp, (M) megakaryocytes, (arrow) lymphoid necrosis, (curved arrow) lymphoid depletion, (arrowhead) congestion, (wavy arrow) perifollicular hemorrhage, and (asterisk) hemorrhage in red pulp. CSNPs: Chitosan nanoparticles-treated group; Cur-CSNPs: curcumin-loaded Chitosan nanoparticles-treated group.

Article Snippet: Spiramycin® tablets (Rovamycin® -Sanofi Aventis Pharmaceutical Company, France) were weighed and crushed into powder.

Techniques: Staining, Negative Control, Infection

Immuno-expression of IL-6 in brain, liver, and spleen tissues. The anti-IL-6 antibodies demonstrate a strong and extensive, brown-colored expression in brain, liver, and spleen of the infected-untreated mice compared to their moderate expression in Spiramycin- and CSNPs- treated groups and weak expression in negative control and Cu-CSNPs-treated group. CSNPs: Chitosan nanoparticles-treated group; Cur-CSNPs: curcumin-loaded Chitosan nanoparticles-treated group.

Journal: Scientific Reports

Article Title: Exploring the role of chitosan and curcumin-loaded chitosan nanoparticles against chronic toxoplasma infection in experimental mice

doi: 10.1038/s41598-025-25252-5

Figure Lengend Snippet: Immuno-expression of IL-6 in brain, liver, and spleen tissues. The anti-IL-6 antibodies demonstrate a strong and extensive, brown-colored expression in brain, liver, and spleen of the infected-untreated mice compared to their moderate expression in Spiramycin- and CSNPs- treated groups and weak expression in negative control and Cu-CSNPs-treated group. CSNPs: Chitosan nanoparticles-treated group; Cur-CSNPs: curcumin-loaded Chitosan nanoparticles-treated group.

Article Snippet: Spiramycin® tablets (Rovamycin® -Sanofi Aventis Pharmaceutical Company, France) were weighed and crushed into powder.

Techniques: Expressing, Infection, Negative Control

Immuno-expression of TNF-α in brain, liver, and spleen tissues. The anti-TNF-α antibodies demonstrate a strong and extensive, brown-colored expression in brain, liver, and spleen of the infected-untreated mice compared to their moderate expression in Spiramycin- and CSNPs- treated groups and weak expression in control negative and Cu-CSNPs-treated group. CSNPs: Chitosan nanoparticles-treated group; Cur-CSNPs: curcumin-loaded Chitosan nanoparticles-treated group.

Journal: Scientific Reports

Article Title: Exploring the role of chitosan and curcumin-loaded chitosan nanoparticles against chronic toxoplasma infection in experimental mice

doi: 10.1038/s41598-025-25252-5

Figure Lengend Snippet: Immuno-expression of TNF-α in brain, liver, and spleen tissues. The anti-TNF-α antibodies demonstrate a strong and extensive, brown-colored expression in brain, liver, and spleen of the infected-untreated mice compared to their moderate expression in Spiramycin- and CSNPs- treated groups and weak expression in control negative and Cu-CSNPs-treated group. CSNPs: Chitosan nanoparticles-treated group; Cur-CSNPs: curcumin-loaded Chitosan nanoparticles-treated group.

Article Snippet: Spiramycin® tablets (Rovamycin® -Sanofi Aventis Pharmaceutical Company, France) were weighed and crushed into powder.

Techniques: Expressing, Infection, Control